Other
Part:BBa_K3365011:Design
Designed by: JUNYAN LI Group: iGEM20_SJTU-BioX-Shanghai (2020-10-23)
sgRNA production module
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 30
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal SpeI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 30
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 30
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
None
Source
The target sequence is from the reference and the fragment is synthesized by the company.
References
[1] Lu Y, Xue J, Deng T, Zhou X, Yu K, Deng L, Huang M, Yi X, Liang M, Wang Y, Shen H, Tong R, Wang W, Li L, Song J, Li J, Su X, Ding Z, Gong Y, Zhu J, Wang Y, Zou B, Zhang Y, Li Y, Zhou L, Liu Y, Yu M, Wang Y, Zhang X, Yin L, Xia X, Zeng Y, Zhou Q, Ying B, Chen C, Wei Y, Li W, Mok T. Safety and feasibility of CRISPR-edited T cells in patients with refractory non-small-cell lung cancer. Nat Med. 2020 May;26(5):732-740.